1d11 pan tgfβ neutralizing antibody  (Bio X Cell)

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Elevated TGFβ signaling contributes to ocular anterior segment dysgenesis in Col4a1 mutant mice

doi: 10.1016/j.matbio.2022.05.001

Figure Lengend Snippet: (A) Schematic illustration of the 1D11 administration paradigm for histological analysis. (B) Representative images of H&E stained sections (left) showing whole eyes (top panels) and corneas (lower panels) from E18.5 mice and quantification graph (right) showing increased corneal stromal thickness in Col4a1+/G1344D mice treated with 1D11 compared to those that received the control IgG1 antibody. Scale bars = 200 μm (top) and 50 μm (bottom). n = 20 and 24 corneas from IgG1- and 1D11-treated Col4a1+/+ mice, and 24 and 30 corneas from IgG1- and 1D11-treated Col4a1+/G1344D mice, respectively. (C) Schematic illustration of the 1D11 administration paradigm for qPCR analyses. (D) qPCR analyses revealed that 1D11 treatment partially prevented the increased expression of TGFβ target genes in anterior segments from P0 Col4a1+/G1344D mice compared to their IgG1-treated counterparts. n = 5–6 samples per genotype. Data are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001, two-way ANOVA and Tukey’s multiple comparison test.

Article Snippet: Timed-pregnant B6 females crossed with Col4a1 +/ G1344D males were injected intraperitoneally with the 1D11 pan-TGFβ neutralizing antibody (clone 1D11.16.8, BioXCell, West Lebanon, NH) or IgG1 isotype control antibody (clone MOPC-21, BioXCell) diluted in inVivoPure Dilution Buffer (pH 7.0, BioXCell) (20 mg/kg) every other day from E8.5 to E16.5 and animals were harvested at E18.5 or P0 for histological and molecular analyses, respectively.

Techniques: Staining, Control, Expressing, Comparison

Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Article Title: Correlations Between Bone Mechanical Properties and Bone Composition Parameters in Mouse Models of Dominant and Recessive Osteogenesis Imperfecta and the Response to Anti-TGF-β Treatment

doi: 10.1002/jbmr.2997

Figure Lengend Snippet: Micro-CT Parameters of Midshaft Femoral Cortical Bone for WT, Crtap −/− , and Col1a2 +/p.G610C Mice

Article Snippet: As described, ( 10 ) 8-week-old female Crtap −/− and Col1a2 +/p.G610C mice were treated for 8 weeks with the pan-TGF-β neutralizing antibody 1D11 (provided by Sanofi Genzyme, Cambridge, MA, USA); control female Crtap −/− , Col1a2 +/p.G610C , and WT mice were treated with a control antibody (13C4) of the same IgG1 isotype that does not inhibit TGF-β.

Techniques: Micro-CT

Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Article Title: Correlations Between Bone Mechanical Properties and Bone Composition Parameters in Mouse Models of Dominant and Recessive Osteogenesis Imperfecta and the Response to Anti-TGF-β Treatment

doi: 10.1002/jbmr.2997

Figure Lengend Snippet: (A) The extrinsic mechanical parameters maximum load, stiffness, post-yield energy, and post-yield displacement of femurs from WT, OI control, and anti-TGF-β-treated (OI anti-TGF-β) mice are shown for the Crtap−/− and Col1a2+/p.G610C models. (B) The intrinsic mechanical parameters ultimate strength, elastic modulus, post-yield toughness, and post-yield strain of femurs of WT, OI control, and OI anti-TGF-β mice are shown for the Crtap−/− and Col1a2+/p.G610C models. *p < 0.05. The mechanical parameters of femurs of Crtap−/− mice are parts of the data set included in an earlier publication(10) and are reproduced here for ease in comparing to the data from the Col1a2+/p.G610C model.

Article Snippet: As described, ( 10 ) 8-week-old female Crtap −/− and Col1a2 +/p.G610C mice were treated for 8 weeks with the pan-TGF-β neutralizing antibody 1D11 (provided by Sanofi Genzyme, Cambridge, MA, USA); control female Crtap −/− , Col1a2 +/p.G610C , and WT mice were treated with a control antibody (13C4) of the same IgG1 isotype that does not inhibit TGF-β.

Techniques:

Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Article Title: Correlations Between Bone Mechanical Properties and Bone Composition Parameters in Mouse Models of Dominant and Recessive Osteogenesis Imperfecta and the Response to Anti-TGF-β Treatment

doi: 10.1002/jbmr.2997

Figure Lengend Snippet: Bone compositional parameters mineral-to-collagen ratio, carbonate substitution, collagen content, and hydroxyapatite crystallinity determined by Raman spectroscopy of femurs are shown for WT, OI control, and OI anti-TGF-β mice for the Crtap−/− and Col1a2+/p.G610C models. *p < 0.05.

Article Snippet: As described, ( 10 ) 8-week-old female Crtap −/− and Col1a2 +/p.G610C mice were treated for 8 weeks with the pan-TGF-β neutralizing antibody 1D11 (provided by Sanofi Genzyme, Cambridge, MA, USA); control female Crtap −/− , Col1a2 +/p.G610C , and WT mice were treated with a control antibody (13C4) of the same IgG1 isotype that does not inhibit TGF-β.

Techniques: Raman Spectroscopy

Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Article Title: Correlations Between Bone Mechanical Properties and Bone Composition Parameters in Mouse Models of Dominant and Recessive Osteogenesis Imperfecta and the Response to Anti-TGF-β Treatment

doi: 10.1002/jbmr.2997

Figure Lengend Snippet: Pearson Correlation Coefficients Between the Mechanical Properties and the Raman Imaging Data for the Crtap −/− Model

Article Snippet: As described, ( 10 ) 8-week-old female Crtap −/− and Col1a2 +/p.G610C mice were treated for 8 weeks with the pan-TGF-β neutralizing antibody 1D11 (provided by Sanofi Genzyme, Cambridge, MA, USA); control female Crtap −/− , Col1a2 +/p.G610C , and WT mice were treated with a control antibody (13C4) of the same IgG1 isotype that does not inhibit TGF-β.

Techniques: Imaging

Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Article Title: Correlations Between Bone Mechanical Properties and Bone Composition Parameters in Mouse Models of Dominant and Recessive Osteogenesis Imperfecta and the Response to Anti-TGF-β Treatment

doi: 10.1002/jbmr.2997

Figure Lengend Snippet: Pearson Correlation Coefficients Between the Mechanical Properties and the Raman Imaging Data for the Col1a2 +/p.G610C Model

Article Snippet: As described, ( 10 ) 8-week-old female Crtap −/− and Col1a2 +/p.G610C mice were treated for 8 weeks with the pan-TGF-β neutralizing antibody 1D11 (provided by Sanofi Genzyme, Cambridge, MA, USA); control female Crtap −/− , Col1a2 +/p.G610C , and WT mice were treated with a control antibody (13C4) of the same IgG1 isotype that does not inhibit TGF-β.

Techniques: Imaging